Contains live spores of the vaccine strain STI-1, freeze-dried in a 10% sucrose solution. Named after the Sanitary Engineering Institute where it was developed. To produce the vaccine, a persistent variant (mutant) of anthrax bacteria is used, devoid of a capsule and the ability to cause diseases in humans and animals. The drug is used for the prevention of anthrax for epidemiological reasons in persons aged 14 to 60 years. Vaccination is carried out twice with an interval of 21 days, revaccination is carried out annually, once. Vaccination is carried out for persons involved in the collection, storage, transportation and processing of raw materials of animal origin, as well as for personnel involved in slaughter, cutting of carcasses and skinning. The drug is administered cutaneously or subcutaneously.
Anthrax vaccine combined liquid for subcutaneous use.
The drug is a mixture of live spores of the STI-1 vaccine strain of the anthrax microbe and purified, concentrated, protective anthrax antigen (PA) adsorbed on aluminum hydroxide. The vaccine is used to prevent anthrax in people aged 14 to 60 years. Vaccination is carried out once, the first three revaccinations are carried out with an interval of 1 year, the next three - with an interval of 2 years. Intense immunity is formed 7 days after the first administration of the vaccine and persists for a year. The drug is administered subcutaneously.
Equine anti-anthrax globulin liquid.
Contains antibodies against the causative agent of anthrax. When receiving the drug, horses are hyperimmunized, then from blood serum by ethanol precipitation with low temperatures fractions of gamma and beta globulins are isolated. Contains no preservatives. Used for emergency prevention and treatment of anthrax patients. The drug is administered intramuscularly. For prophylactic purposes, it is administered once (as soon as possible after suspected infection), for therapeutic purposes - immediately after the diagnosis of anthrax is established for 3 - 4 days. Before using the drug, an intradermal test is necessary. If the test is positive, globulin is administered only according to vital signs under the supervision of a doctor.
Drugs for the prevention and treatment of staphylococcal infections
Purified adsorbed staphylococcal toxoid.
Contains 10 EC of staphylococcal toxoid per ml. When preparing the drug, staphylococcal toxin (contained in the filtrate of a staphylococcus broth culture) is neutralized with formaldehyde and heat, precipitated with trichloroacetic acid, purified with ethyl alcohol and adsorbed on aluminum hydroxide. When an toxoid is introduced into the body, it induces the formation of antitoxic antibodies. Used for prevention staphylococcal infections. The drug is administered:
a) workers at high risk of injury: b) patients undergoing planned operations: c) pregnant women: d) donors to obtain anti-staphylococcal plasma and anti-staphylococcal immunoglobulin. Anatoxin is administered subcutaneously, usually twice with an interval of 20 - 40 days.
Staphylococcal vaccine.
Contains a suspension of staphylococci in a 0.9% aCl solution, inactivated by heating for 2 hours at 560 C. The vaccine is prepared from 10 - 12 coagulase-positive strains of staphylococcus isolated from patients. Preservative - phenol. The drug is used to treat adults with various diseases staphylococcal and streptostaphylococcal etiology: recurrent furunculosis, pyoderma, panaritium, acne, etc. The vaccine is administered subcutaneously or intramuscularly.
Staphylo-Protean-Pseudomonas aeruginosa vaccine adsorbed liquid.
It is a complex of purified, concentrated toxoids of staphylococcus and Pseudomonas aeruginosa, cytoplasmic antigen of staphylococcus and chemical proteus vaccine, adsorbed on aluminum hydroxide. The drug is used for active immunization of patients aged 18 - 60 years to prevent infections caused by staphylococci, Proteus and Pseudomonas aeruginosa. The vaccine should be administered to patients with injuries, burns, fractures, as well as during preoperative preparation.
Vaccine to prevent anthrax
Live anthrax vaccine: Pharmacological action
After two-time use with an interval of 20-30 days, it causes the formation of intense immunity lasting up to 1 year.
Live anthrax vaccine: Indications
Specific prevention of anthrax: persons working with live cultures of the anthrax pathogen, with infected laboratory animals or conducting research on materials contaminated with the anthrax pathogen; persons slaughtering livestock, engaged in the procurement, collection, storage, transportation, processing and sale of raw materials of animal origin; persons performing work in areas enzootic for anthrax (maintenance of public livestock, agricultural, agro- and land reclamation, construction and other work related to the excavation and movement of soil, procurement, fishing, geological, survey, expeditionary work).
Live anthrax vaccine: Contraindications
Acute (infectious and non-infectious) diseases (vaccination is carried out no earlier than 1 month after recovery or remission), immunodeficiency (primary and secondary), simultaneous use of corticosteroids, antimetabolites, radiation therapy(vaccination is carried out no earlier than 6 months after the end of therapy), malignant neoplasms and malignant blood diseases, systemic diseases connective tissue, common recurrent skin diseases, diseases endocrine system, pregnancy, lactation period, childhood(up to 14 years old).
Live anthrax vaccine: Side effects
When applied cutaneously, a local reaction appears after 24-48 hours: hyperemia, a slight infiltrate, followed by the formation of a yellowish crust along the incisions. With syringe and needleless methods of administration, after 24-48 hours at the injection site there is pain, hyperemia, and less often - an infiltrate with a diameter of up to 50 mm. A general reaction during cutaneous and subcutaneous administration of the vaccine rarely occurs in the first day after administration: malaise, headache, slight increase in temperature. Sometimes there may be an increase in body temperature up to 38.5 degrees C and a slight increase in regional lymph nodes.
Live anthrax vaccine: Method of administration and dose
Cutaneously (scarification) and subcutaneously. It is advisable to carry out unscheduled vaccination subcutaneously. Primary immunization is carried out twice with an interval of 20-30 days, revaccination is carried out once annually. A cutaneous dose of the vaccine is 0.05 ml (contains 500 million spores), one subcutaneous dose is 0.5 ml (50 million spores). Cutaneously (scarification): the contents of the ampoule immediately before use are resuspended in sterile 30% aqueous solution glycerol, which is added to the ampoule using a syringe with a needle for intramuscular administration. The volume of solvent is determined by the number of vaccination doses in the ampoule. Add 0.5 ml to an ampoule with 10 cutaneous doses, and 1 ml of solvent into an ampoule with 20 cutaneous doses. The ampoule is shaken until a homogeneous suspension is formed. The dissolution time of the vaccine should not exceed 5 minutes. The diluted vaccine from an opened ampoule, stored under aseptic conditions, can be used within 4 hours. Vaccination is carried out on outer surface middle third shoulder The vaccination site is treated with ethanol or a mixture of ethanol and ether. Application of other disinfectant solutions not allowed. After the ethanol and ether have evaporated, use a sterile tuberculin syringe with a thin and short needle (N 0415), without touching the skin, apply one drop (0.025 ml) of the diluted vaccine to 2 places of future incisions at a distance of 3-4 cm. The skin is slightly stretched and sterile Using a smallpox vaccination pen, make 2 parallel cuts 10 mm long through each drop of vaccine so that they do not bleed (blood should appear only in the form of small ones). Using the flat side of a smallpox vaccination feather, rub the vaccine into the incisions for 30 seconds and allow to dry for 5-10 minutes. A separate disposable feather is used for each person being vaccinated. It is prohibited to use needles, scalpels, etc. instead of pens. S.C.: Immediately before use, the vaccine is resuspended in 1 ml of a sterile 0.9% NaCl solution. The ampoule is shaken until a uniform suspension is formed. The contents of the ampoule are transferred with a sterile syringe into a sterile bottle with 0.9% NaCl solution. In the case of using an ampoule containing 200 subcutaneous vaccination doses, the suspension is transferred to a bottle with 99 ml, and one containing 100 subcutaneous vaccination doses - into a bottle with 49 ml of solvent. With the syringe method, the vaccine is injected into the area of the lower corner of the scapula in a dose of 0.5 ml. The skin at the injection site is treated with ethanol or a mixture of ethanol and ether. A disposable syringe and needle are used for each person vaccinated. Before each vaccine collection, the vial is shaken. The injection site is lubricated with 5% tincture of iodine. When applying the vaccine subcutaneously without a needle, the vaccine is administered in a volume of 0.5 ml to the outer surface area upper third shoulder with a needleless injector with a protector, strictly following the instructions for their use. The vaccine injection site is treated before and after injection, as with the syringe method.
Live anthrax vaccine:: Special instructions
It is strictly forbidden to administer subcutaneously a vaccine diluted for cutaneous use! In order to identify contraindications, the doctor (paramedic) on the day of vaccination conducts a survey and examination of the vaccinated with mandatory thermometry. In every special case For diseases not included in this list of contraindications, vaccination is carried out only with the permission of the relevant medical specialist. Vaccination is routinely carried out by the cutaneous method in the first quarter of the year, because The most dangerous period in terms of anthrax infection in disadvantaged areas is the spring-summer season. Vaccination is carried out by nursing staff under the guidance of a doctor. Before use, each ampoule of vaccine is carefully inspected. The vaccine cannot be used if the integrity of the ampoule is damaged or if the appearance dry and dissolved drug (foreign particles, unbroken lumps and flakes), lack of label, expired, in violation of storage conditions. Unused vaccine, used vaccination disposable syringes and feathers are subject to mandatory inactivation by autoclaving at a temperature of (132+2) degrees C and a pressure of 2 kG/sq.m for 90 minutes. Parts of the needleless injector that came into contact with the vaccine after pre-treatment immersed in a 6% solution of hydrogen peroxide with 0.5% detergent type or for 1 hour at a temperature not lower than 50 degrees C. The solution is used once. Parts of the injector are sterilized by autoclaving at a temperature of (132+2) degrees C and a pressure of 2 kgf/sq.m for 90 minutes.
Live anthrax vaccine:: Interaction
The interval between vaccination against anthrax and the administration of other vaccines should be at least 1 month.
If the anthrax vaccine is not given, a person can become infected by eating the meat of an affected animal, so it is. Anthrax is a particularly dangerous pathology of infectious origin. If a person becomes infected, there is incubation period, then carbuncles form on the surface of the dermis. The disease spreads through contact. To avoid contamination, you should purchase meat products from quality suppliers.
The first symptoms may appear after 4 days. To make a diagnosis, you need to examine the sputum and exudate that separates from the surface of the skin, then the doctor prescribes other examinations. Medicines are used for treatment penicillin series. Anthrax is caused by the rod-shaped bacterium Bacillus anthracis.
Clinical manifestations
The incubation period lasts 4 days (sometimes up to 2 hours). The carbunculous form of the disease is common among people. IN in this case a pea-sized formation forms on the skin. At first it looks like a reddish spot, then it turns into a papule that rises above the surface of the skin. A sign of anthrax is itchy skin.
As the pathology progresses, the papule becomes filled with serous contents and slightly enlarges. Later it acquires a dark color. After a few days, a black scab forms on the surface, the formation becomes crust-like, and redness and swelling are localized around it. If the formation is located on the cheeks or neck, it can lead to damage respiratory system, then suffocation occurs. The pathology is accompanied by intoxication, the person feels unwell and feels aching muscles.
The pathology occurs against the background of fever. A few days after infection, a decrease in temperature is observed, and the symptoms subside. After 15 days, the formations disappear, leaving a scar on the skin. In exceptional cases, several carbuncles are formed. The danger is posed by those that have formed on the head; in this case, there is a high probability of suffocation and sepsis. Timely consultation with a doctor significantly improves life prognosis.
Some are diagnosed with the ideomotor form of the disease. The pathology is accompanied by tissue hyperemia and the formation of carbuncles. The generalized form of the pathology leads to damage to the respiratory system. Symptoms can be confused with ARVI. In the generalized form, intoxication, runny nose, and cough occur. Characteristic sign- tachycardia. After 2 hours, the temperature rises to critical levels, the patient feels unbearable pain in the sternum. Wet cough contains blood clots. Subsequently, the activity of the heart is disrupted.
Intestinal anthrax is extremely dangerous and the pathology is fatal. First, fever occurs, then intoxication. A person feels severe pain in the throat, their duration is up to 2 days. The disease leads to nausea. A symptom of an intestinal type of disease is vomiting with blood clots, the patient also has diarrhea. The progression of pathology leads to disturbances in cardiovascular system. The face takes on a bluish color, and a hemorrhagic rash appears on the surface of the skin. The septic form of anthrax leads to death.
The pathology can occur against the background of the disease “Meningitis”. The progression of anthrax leads to meningoencephalitis, swelling of the cerebral cortex. Other dangerous complications:
- pulmonary edema;
- asphyxia;
- bleeding in the gastrointestinal tract.
- Anthrax can lead to sepsis and shock.
Diagnostic measures
To confirm anthrax, diagnostics is carried out in several stages. Biological materials are examined, bacterial cultures are performed, and serological tests are carried out. An X-ray of the lungs is required to confirm the diagnosis. There may be Clinical signs pneumonia or pleurisy disease. If necessary, the patient consults a pulmonologist; the doctor may prescribe a pleural puncture. On initial stages requires examination by a dermatologist.
The doctor prescribes penicillin medications, which, as well as other serums, are administered intravenously. Duration of use - 7 days. The drugs help eliminate the symptoms of intoxication. The anthrax vaccine for humans is Doxycycline. To suppress the pathogen, injections of ciprofloxacin are used. Next, therapy is carried out to prevent intoxication. Drugs containing Prednisolone are administered. If the pathology leads to dangerous complication, is appointed intensive treatment. For elimination skin manifestations special dressings are applied. Anthrax cannot be treated with surgery.
The disease has a different prognosis. If the skin form of the disease has been diagnosed, the prognosis is good. Generalized types are not fatal. At timely treatment the prognosis will be improved. It is important to comply with sanitary and hygienic standards; they will ensure the prevention of anthrax. It is necessary to process animal raw materials in a timely manner and store them correctly. It is important to follow the rules for transporting and burying affected livestock.
It is required to adhere to sanitary and hygienic rules when working with livestock. If there is a risk of infection, you need to get an anthrax vaccine, this way you will be able to protect yourself from the disease. Vaccination is carried out in clinics; after the procedure, you must follow the doctor’s recommendations.
Experiments and how the anthrax vaccine is distributed
Who created the vaccine to combat anthrax? The scientist's name is Louis Pasteur. The bacterium that causes the disease was discovered at the end of the 19th century and was bred by Robert Koch. Louis Pasteur founded the experiment, which demonstrated the effect of the vaccine. The researchers took 50 sheep and divided them into two groups. One group was vaccinated, the second was not. A month later, the sheep were given a vaccine containing live cultures. Sheep that received the anthrax vaccine survived, while others died.
In 1954, specialists developed a vaccine for humans. It became available in the early seventies. Today the vaccine is produced in dry form and is used for subcutaneous administration. The certified product contains active substances with glycerin. If a person is at risk of becoming infected, it is necessary to get vaccinated.
Vaccination is needed for laboratory technicians who come into contact with sick people, veterinarians, and people working in enterprises. Vaccination is required for persons working in slaughterhouses. In the early nineties, 25 million people were vaccinated. Researchers are confident that if the drug is available in every medical institution, the risk of the spread of pathology will decrease.
The vaccine is intended for animals older than three months. The drug is produced in specialized bottles and stored under optimal conditions. Anthrax vaccine is a transparent white, homogeneous liquid.
Shelf life of the drug, storage conditions
Instructions for use contain information that the drug contains anthrax spores of a capsular virulent culture. The active ingredients are mixed with a glycerin solution. The vaccine is available in different bottles, depending on the animal’s body weight; the veterinarian chooses a volume of 20, 50 or 100 ml. One milliliter of the active ingredient includes 20 million spores. Dry live anthrax vaccine is administered by an experienced veterinarian. Independent use is prohibited! The bottle contains information about the manufacturer, the data of the packer, as well as the time of creation. The instructions contain dosages and storage conditions.
According to established rules, the drug is supplied to pharmacies in wooden boxes (capacity of 1 box is 15 kg). These boxes contain a control document with complete information about the drug. If transportation is planned, it is necessary to create optimal conditions. The anthrax vaccine is transported at a temperature of + 15 degrees. The drug is not stored in conditions where the air temperature is below 0. The vaccine is stored for 2 years. If deviations from generally accepted standards are detected, the drugs are destroyed. If necessary, batches or individual medicinal formulations are rejected.
Application of vaccine for animals
The drug is used for prophylactic purposes. The dosage varies. Intramuscular administration active substance requires compliance with rules. If they are violated, side effects will occur that will lead to the death of the animal. The drug is administered if the animal is already 3 months old; therefore, the contraindication is childhood. The vaccine can be used to vaccinate goats and sheep. The doctor inserts it into the neck or chest. Dosages are strictly individual. If the drug is used for vaccination of cattle, the dose is increased. The injection area is behind the ear or near the thigh.
Before administering the drug, you should disinfect skin covering. For these purposes, alcohol or a weak solution of phenol is used. The vaccine is administered with a high-quality syringe; you need to choose one on which the needles will stick well. The needles should not be short, a suitable length is 15 mm. Before introducing the composition, the instruments should be disinfected by treating boiled water. Sterilization is necessary to prevent infection.
After use, the instruments are boiled with a weak soda solution. Boiling time is one hour. Shake before administration medicinal composition, you should get a liquid of uniform color. If the bottle has been opened but the veterinarian has not used the formulation, it should be thrown away. Liquid anthrax vaccine is used veterinarian, the animal is first examined.
If signs of any disease are detected, vaccination is postponed and done after the animal has recovered. Administration is contraindicated when elevated temperature, weakness, general malaise. The drug can be used to vaccinate pregnant females, but only if absolutely necessary. If you follow the rules for using the medicine, there will be no side effects.
Veterinarians do not recommend vaccinations in summer and in cold weather. The weather should be moderately warm. After vaccination, it is necessary to carefully monitor the animal; it should not be exposed to stress or hypothermia. It is necessary to ensure proper rest. If the vaccine is given to horses, they are suspended from work for a week. It is important to comply with the timing of vaccination; the drug is administered once every 12 months.
The doctor may prescribe emergency vaccination. In this case, the medicine can be administered if the animal's condition is satisfactory.
If it is necessary to vaccinate lambs and kids that are 3 months old, re-vaccination is carried out upon reaching one year. The vaccine is used to prevent the disease in calves that have reached three months of age; after six months, immunization of adult livestock is required. Immunization of foals is carried out for the first time at the age of 9 months, then upon reaching maturity.
The drug is administered to horses once, at a time when the body is completely healthy. When a camel is three months old, immunization is required, then once a year.
Forced vaccinations are carried out regardless of the time of year. There are times when it is necessary to immunize an animal with an infectious pathology. However, if a particular disease is very acute and there is a significant increase in temperature, the timing of vaccination is reconsidered. In some cases, the drug is administered after treatment of the disease. The vaccine from strain 55 cannot be combined with others, or with drugs that have a similar mechanism of action.
For ten days after administration, the animal’s skin cannot be disinfected. Serums are not administered intravenously to treat diseases. The animal's health is monitored by a veterinarian. After twelve days, resistance to the anthrax bacterium is formed. The maximum duration of immunity is 11 months. Some animals exhibit hypersensitivity to the vaccine. Swelling appears in the injection area, the temperature may rise, and chills may occur. The swelling goes away after two days, and fever is rare. Side effects possible if the rules for introducing the composition were violated.
Dosage form: lyophilisate for preparing a suspension for subcutaneous administration and cutaneous scarification Compound:
Live anthrax vaccine, lyophilisate for preparing a suspension for subcutaneous administration and cutaneous scarification, is a lyophilized suspension of live spores of the vaccine strain Bacillus anthracis STI-1.
Stabilizer - 10% sucrose solution.
Description: A porous mass of grayish-white or yellowish-white color with a brownish tint. Pharmacotherapeutic group: MIBP - ATX vaccine:J.07.A.C Vaccine to prevent anthrax
J.07.A.C.01 Anthrax antigen
Pharmacodynamics:Live anthrax vaccine after two-time use with an interval of 20-30 days causes the formation specific immunity lasting up to 1 year.
Indications:Specific prevention of anthrax in people over 14 years of age. Vaccination is carried out as planned and according to epidemic indications.
The following are subject to routine vaccinations:
Persons working with live cultures of the causative agent of anthrax, with infected laboratory animals, or conducting research on materials contaminated with the causative agent of anthrax;
Persons who slaughter livestock, engaged in the procurement, collection, storage, transportation, processing and sale of raw materials of animal origin;
Persons performing the following work in anthrax-enzootic areas:
Public livestock maintenance;
Agricultural, agro-and drainage, construction and other work related to the excavation and movement of soil;
Procurement, fishing, geological, survey, expedition.
Vaccination is routinely carried out in the first quarter of the year, because The most dangerous period in terms of anthrax infection in disadvantaged areas is the spring-summer season.
Contraindications:1. Acute infectious and non-communicable diseases- vaccinations are carried out no earlier than 1 month after recovery (remission).
2. Primary and secondary immunodeficiencies. When treating with steroids, antimetabolites, or radiotherapy, vaccinations are carried out no earlier than 6 months after the end of therapy.
3. Malignant neoplasms and malignant blood diseases.
4. Systemic connective tissue diseases.
5. Common recurrent skin diseases.
6. Diseases of the endocrine system.
7. Pregnancy and lactation.
In each individual case, for diseases not included in this list, vaccination is carried out only by permission. the relevant medical specialist.
In order to identify contraindications, the doctor (paramedic) on the day of vaccination conducts a survey and examination of the vaccinated with mandatory thermometry.
Directions for use and dosage:Vaccination is carried out by nursing staff under the guidance of a doctor.
Routine vaccination. Primary immunization is carried out by scarification twice with an interval of 20-30 days, revaccination is carried out once annually by subcutaneous method.
Vaccination according to epidemic indications carried out subcutaneously. If necessary, revaccination is carried out once annually subcutaneously.
Before use, each ampoule of vaccine is carefully inspected. The vaccine cannot be used if the integrity of the ampoule is damaged, the appearance of the dry and dissolved drug changes (presence of foreign inclusions, unbreakable lumps and flakes), the label is missing, the expiration date has expired, or storage conditions have been violated.
1. Vaccination by cutaneous (scarification) method. Based on the number of vaccination doses, the contents of the ampoule (vial) immediately before use are resuspended in a solvent - a sterile 30% aqueous solution of glycerol using a syringe with a needle for intramuscular injection(No. 0840). Add 0.5 ml to an ampoule (bottle) with 10 cutaneous doses, and 1.0 ml of solvent with 20 cutaneous doses and shake until a homogeneous suspension of grayish-white or yellowish-white color with a brownish tint is formed. The dissolution time of the vaccine should not exceed 5 minutes. The diluted vaccine, stored under aseptic conditions, can be used within 4 hours.
The grafting is carried out on the outer surface of the middle third of the shoulder. The grafting site is treated with 70% alcohol. The use of other disinfectant solutions is not permitted. After the alcohol has evaporated, use a sterile tuberculin syringe with a thin and short needle (No. 0415), without touching the skin, apply one drop (0.025 ml) of the diluted vaccine to 2 places of future incisions at a distance of 3-4 cm on the horizontal surface of the shoulder. The skin is slightly stretched and with a sterile smallpox vaccination pen, 2 parallel cuts are made through each drop of vaccine (at a distance of 3-5
mm) length 10 mm in such a way that they do not bleed (blood can only appear in the form of small dewdrops). Using the flat side of a smallpox vaccination feather, rub the vaccine into the incisions for 30 seconds and allow to dry for 5-10 minutes. A separate disposable feather is used for each person being vaccinated.It is prohibited to use needles, scalpels, etc. instead of feathers.
2. Subcutaneous vaccination.
Immediately before use, the drug is resuspended in 1 ml of sterile sodium chloride solution of 0.9%. The ampoule (bottle) is shaken until a uniform suspension of grayish-white or yellowish-white color with a brownish tint is formed. The contents of the ampoule (vial) are transferred with a sterile syringe into a sterile vial with sodium chloride solution 0.9% for injection. In the case of using an ampoule (bottle) containing 200 subcutaneous vaccination doses, the suspension is transferred to a bottle with 99 ml, and containing 100 subcutaneous vaccination doses - into a bottle with 49 ml of solvent.
With the syringe method the vaccine in a volume of 0.5 ml is injected subcutaneously into the area of the lower angle of the scapula. The skin at the injection site is treated 70% alcohol. Before each vaccine collection, the vial is shaken. The injection site is lubricated with 5% tincture of iodine.
With the needleless method the vaccine in a volume of 0.5 ml is administered into the area of the outer surface of the upper third of the shoulder using a needle-free injector with a protector, strictly following the instructions for its use. The vaccine injection site is treated before and after injection, as with the subcutaneous method.
Unused vaccine, used vaccination disposable syringes and feathers are subject to mandatory inactivation by autoclaving at a temperature of (132±2) °C and a pressure of 2.0 kgf/m2 for 90 minutes.
Parts of the needleless injector that come into contact with the vaccine are immersed in
6 % hydrogen peroxide solution with 0.5% detergent type "Progress" or "Astra" for 1 hour at a temperature not lower than 50 ° C. The solution is used once. Then the injector is pre-sterilized:a) rinsing under running water for 0.5 minutes;
b) soaking at complete immersion into the washing solution at a temperature of 50 °C for 15 minutes. Recipe for 1 liter of washing solution: 17 g perhydrol (27.5 g 33
% hydrogen peroxide), 5 g of detergent and 978 ml of water;c) washing each item in a washing solution using a brush or a cotton-gauze swab for 0.5 minutes;
d) rinsing under running water for 10 minutes;
e) rinsing each item with distilled water for 0.5 minutes;
f) drying until moisture completely disappears.
Sterilization of parts of a needleless injector is carried out by autoclaving at temperature (132±2) °C and pressure
2.0 kgf/m2 for 90 minutes.Reaction to introduction
Vaccine vaccinations may be accompanied by local reactions, the intensity of which depends on individual characteristics vaccinated. 24-48 hours after cutaneous vaccination, hyperemia and infiltration may occur at the site of vaccine administration, followed by the formation of yellowish crusts along the incisions. 24-48 hours after subcutaneous vaccination, pain, hyperemia, and, less commonly, an infiltrate with a diameter of up to 50 mm may occur at the injection site.
Side effects:It may manifest itself in the first day after vaccination with malaise, headache and an increase in body temperature up to 38.5 ° C.
Interaction: Interval between vaccinesinaziagainst anthrax and other vaccines must be at least one month old, and for children - at least two months.The vaccine is sensitiveToantibiotics, and therefore immunization while using antibiotics is not allowed.
Release form/dosage:Lyophilisate for preparing a suspension for subcutaneous administration and cutaneous scarification. Package:200 subcutaneous or 20 cutaneous vaccination doses in an ampoule (vial) (solvent - 30% glycerol solution, 1.5 ml in an ampoule) or 100 subcutaneous or 10 cutaneous vaccination doses in an ampoule (vial) (solvent - 30% glycerol solution, 1.5 ml each) 0 ml per ampoule).
Cutaneously (scarification) and subcutaneously.It is advisable to carry out unscheduled vaccination subcutaneously.
Primary immunization is carried out twice with an interval of 20-30 days, revaccination is carried out once annually. A cutaneous dose of the vaccine is 0.05 ml (contains 500 million spores), one subcutaneous dose is 0.5 ml (50 million spores).
Cutaneously (scarification): immediately before use, the contents of the ampoule are resuspended in a sterile 30% aqueous solution of glycerol, which is added to the ampoule using a syringe with a needle for intramuscular administration. The volume of solvent is determined by the number of vaccination doses in the ampoule. Add 0.5 ml to an ampoule with 10 cutaneous doses, and 1 ml of solvent into an ampoule with 20 cutaneous doses. The ampoule is shaken until a homogeneous suspension is formed. The dissolution time of the vaccine should not exceed 5 minutes. The diluted vaccine from an opened ampoule, stored under aseptic conditions, can be used within 4 hours. Vaccination is carried out on the outer surface of the middle third of the shoulder. The vaccination site is treated with ethanol or a mixture of ethanol and ether. The use of other disinfectant solutions is not permitted. After the ethanol and ether have evaporated, use a sterile tuberculin syringe with a thin and short needle (No. 0415), without touching the skin, apply one drop (0.025 ml) of the diluted vaccine to 2 places of future incisions at a distance of 3-4 cm. The skin is slightly stretched and sterile Using a smallpox vaccination pen, 2 parallel cuts 10 mm long are made through each drop of vaccine so that they do not bleed (blood should appear only in the form of small “dewdrops”). Using the flat side of a smallpox vaccination feather, rub the vaccine into the incisions for 30 seconds and allow to dry for 5-10 minutes. A separate disposable feather is used for each person being vaccinated. It is prohibited to use needles, scalpels, etc. instead of pens.
S.C.: Immediately before use, the vaccine is resuspended in 1 ml of a sterile 0.9% NaCl solution. The ampoule is shaken until a uniform suspension is formed. The contents of the ampoule are transferred with a sterile syringe into a sterile vial with 0.9% NaCl solution. In the case of using an ampoule containing 200 subcutaneous vaccination doses, the suspension is transferred to a bottle with 99 ml, and one containing 100 subcutaneous vaccination doses - into a bottle with 49 ml of solvent.
With the syringe method, the vaccine is injected into the area of the lower corner of the scapula in a dose of 0.5 ml. The skin at the injection site is treated with ethanol or a mixture of ethanol and ether. A disposable syringe and needle are used for each person vaccinated. Before each vaccine collection, the vial is shaken. The injection site is lubricated with 5% tincture of iodine.
When using the vaccine subcutaneously using the needle-free method, the vaccine is administered in a volume of 0.5 ml into the area of the outer surface of the upper third of the arm using a needle-free injector with a protector, strictly following the instructions for their use.
The vaccine injection site is treated before and after injection, as with the syringe method.
